Fig. 4.

KR33426 reduced c-Fos leading to a decrease in BAFF expression. (A) MH7A cells were treated with TNF-α in the presence or absence of KR33426. Cell lysates were prepared and western blotting was used to detect JNK, phospho-JNK, phospho-p38, ERK and phospho-ERK. (B) MH7A cells were treated with TNF-α. Cell lysates were prepared and western blotting was used to detect p65, phospho-p65 (serine 536 or 276), c-Fos, phospho-CREB (serine 133) and SP1. (C) MH7A cells were treated with TNF-α in the presence or absence of KR33426. Cell lysates were prepared and western blotting was used to detect c-Fos. (D) MH7A cells were co-transfected with pGL3-BAFF-Luc plasmids and c-Fos-siRNA. Luciferase activity of BAFF promoter was measured by using luminometer. Data were the representative of four experiments (A-D). Data in the bar graph represent the means ± SED. *p<0.05; **p<0.01, significant difference as compared to pGL3-BAFF-Luc plasmids-untransfected control. &p<0.05; &&p<0.01, significant difference from scambled siRNA-treated control at each DNA concentration of pGL3-BAFF-Luc plasmids.