Fig. 4.

Dasatinib blockades phosphorylation of Syk and down-signaling protein in antigen-stimulated MCs. (A) RBL-2H3 cells and (B) BMMCs were incubated overnight with antigen-specific IgE. The cells were stimulated with 50 ng/ml antigen for 15 min with or without the pre-treating dasatinib or PP2 for 30 min, as indicated. The cell lysates were used in the Western blot analysis as described in the section for “Materials and Methods”. Representative images (A, B) or the mean ± SEM of band density (C, the upper for panel A; the lower for panel B) from three-independent experiments are shown. Asterisks indicate significant differences between the values obtained following antigen stimulation without dasatinib and those obtained following antigen stimulation with dasatinib or PP2. *p<0.05 and **p<0.01.