Figure 3.

Nonspecific killing (NSK) and inhibition of phagocytosis. A, NSK of various bacterial strains in the presence of different baby rabbit serum (BRS) concentrations. TIGR-JS and R36A are nonencapsulated control strains. FG34 and FG44 are TIGR-JS transformants with D39 cps and SK95 cps, respectively. SPEC1, TIGR4, and TREP19A are encapsulated pneumococcal isolates [33], and their killing curves lie between FG34 and SPEC6B, and are not shown for the purpose of clarity. B, NSK of bacterial isolates (labeled on x-axis) with HL60 cells treated with OBB alone (white bar), OBB with 10 µmol/L cytochalasin D (Cyto-D) (black bar), or OBB with 1% dimethyl sulfoxide (DMSO) control (gray bar) at 10% BRS. Error bars indicate means of 3 replicates with standard errors. *P < .001.