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. 2020 Aug 27;20:414. doi: 10.1186/s12935-020-01510-6

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Knockdown of MIR99AHG suppressed GC cell proliferation, invasion and migration in vitro. a MIR99AHG expression was measured by qPCR. b, c Growth curves were measured by CCK-8 assay. d Trans-well assay; upper panel shows migration effect and lower panel shows invasion effect. e, f Qualification of the trans-well assay. g E-cadherin and N-cadherin expression levels were detected by Western blotting. h, i Knockdown MIR99AHG promoted apoptosis in GC cells. Representative FACS images and statistics based on three independent experiments. j Apoptosis related key proteins were detected by Western blotting. *p < 0.05, **p < 0.01 and ***p < 0.001 (two-way ANOVA)