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. 2020 Aug 27;22:199. doi: 10.1186/s13075-020-02295-9

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — S100A8 and S100A9 expression is increased during SCW arthritis. a In synovial washouts, S100A8/9 was detected using an ELISA. Washouts were obtained by washing synovial explants for 2 h in the culture medium at RT. Explants were isolated at baseline and at days 1, 2, and 7 (b). Using immunohistochemistry, S100A8 and S100A9 (c) were detected in the knee joints at baseline and at days 1, 2, and 7 after induction of synovitis. S100A8/9 is clearly increased at days 1 and 2 and wanes thereafter (n = 6 for all groups). d This is quantified using ImageJ. One-way ANOVA with Tukey multiple comparison test was performed. ***p < 0.001; **p < 0.01; *p < 0.05. P, patella; F, femur; S, synovium