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. Author manuscript; available in PMC: 2020 Aug 31.
Published in final edited form as: Cell Rep. 2019 Jul 2;28(1):267–281.e5. doi: 10.1016/j.celrep.2019.05.107

Figure 4. Mmi1 Recruits CPF to Facultative Heterochromatin Islands.

Figure 4.

(A) ChIP-seq analysis of the core CPF subunit Iss1-GFP in the WT (top) or mmi1Δ (bottom). Enrichments at meiotic island loci are shown. DSR motifs are indicated by red triangles.

(B) Chromatin immunoprecipitation combined with microarray (ChIP-chip) enrichment of CFP-Mmi1 in the WT at the indicated meiotic island loci.

(C) Median Iss1-GFP ChIP-seq enrichment over H3K9me island Mmi1 regulon genes (n = 7) versus non-island (n = 21) Mmi1 regulon genes in WT cells as determined by ChIP-seq.

(D) Median Iss1-GFP ChIP-seq enrichment over island Mmi1 regulon genes (n = 7) in WT (green) or mmi1Δ (orange) cells.

(E) Core CPF subunit Iss1FIP1 coimmunoprecipitates with the YTH family protein Mmi1. The purified Iss1-GFP fraction was probed for FLAG-Mmi1.

(F) Colocalization of Mmi1 and CPF in live WT cells expressing CFP-Mmi1 and Iss1-GFP. Also shown are zoomed insets (dashed orange boxes) of 3 representative cells. The scale bar represents 5 μm. PCC was calculated to quantify population-wide colocalization of Mmi1 and Iss1FIP1.

For all median enrichment analyses, the base coordinate ranges of genes were scaled to a common length from the start codon to the stop codon of the open reading frame (ORF). Island and non-island Mmi1-regulon genes are listed in Table S3.

See also Figure S4 and Table S3.