KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| anti-H3K9me2 | Abcam | ab115159; RRID: AB_10903018 |
| anti-GFP (polyclonal ChIP-grade) | Abcam | ab290; RRID: AB_303395 |
| RNAPII phospho S2 | Abcam | ab5095; RRID: AB_304749 |
| anti-GFP (coupled to agarose beads) | Chromotek | gta20; RRID: AB_2631357 |
| anti-GFP clones 7.1 and 13.1 | Roche | 11814460001; RRID: AB_390913 |
| anti-FLAG M2 | Sigma | F3165: RRID: AB_259529 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Ribo-Zero Gold rRNA Removal kit (Yeast) | Illumina | MRZY1324 |
| iTaq Universal SYBR Green Supermix | BioRad | 1725122 |
| Dimethyl adipimidate (DMA) | Thermo Fisher Scientific | 20660 |
| Formaldehyde, 37% solution | VWR | 97064-604 |
| Protein A/G PLUS-Agarose | Santa Cruz | sc-2003 |
| iTaq Universal SYBR Green Supermix | BioRad | 1725122 |
| N-methyl-N’-nitro-N-nitrosoguanidine | TCI | M0527 |
| PureLink RNase A | Thermo Fisher Scientific | 12091039 |
| Proteinase K solution | Thermo Fisher Scientific | AM2548 |
| Masterpure Yeast RNA purification kit | Lucigen | MPY03100 |
| QIAquick PCR Purification kit | QIAGEN | 28106 |
| MAXIscript T7 Transcription kit | Ambion | AM1312 |
| mirVana miRNA isolation kit | Thermo Fisher Scientific | AM1560 |
| 4-12% Bis-Tris PAGE | Thermo Fisher Scientific | NP0321BOX |
| HybondTM-N+ | Fisher Scientific | 45-000850 |
| α-P32-UTP | Perkin Elmer | BLU007H250uc |
| Critical Commercial Assays | ||
| NEBNext Ultra II DNA library prep kit for Illumina | New England Bio Labs | E7645S |
| ScriptSeq v2 RNA-seq library preparation kit | Epicenter | SSV21124 |
| Deposited Data | ||
| ChIP-seq | This study | GEO: GSE123137 |
| RNA-seq | This study | GEO: GSE123138 |
| Figure 3 WT 30°C | Gallagher et al., 2018 | GEO: GSE104547 |
| Figure S4A Rna14, Pcf11 ChIP-seq | Larochelle et al., 2018 | GEO: GSE115595 |
| Figure S5A Rna14, Pcf11 ChIP-seq | Larochelle et al., 2018 | GEO: GSE115595 |
| Figure S7 Rna14, Pcf11 ChIP-seq | Larochelle et al., 2018 | GEO: GSE115595 |
| Experimental Models: Organisms/Strains | ||
| S. pombe | Grewal lab | Table S4 |
| Oligonucleotides | ||
| ChIP-PCR, ChIP-qPCR, Northern blot probing | This study | Table S5 |
| Software and Algorithms | ||
| Trimmomatic | Bolger et al., 2014 | http://www.usadellab.org/cms/?page=trimmomatic |
| BWA-MEM | Li and Durbin, 2010 | http://bio-bwa.sourceforge.net/bwa.shtml |
| Bcftools | Li, 2011 | https://samtools.github.io/bcftools/bcftools.html |
| SnpEff | Cingolani et al., 2012 | http://snpeff.sourceforge.net/SnpEff_manual.html |
| Rsubread | Liao et al., 2013 | https://bioconductor.org/packages/release/bioc/html/Rsubread.html |
| ComplexHeatmap | Gu et al., 2016 | https://bioconductor.org/packages/release/bioc/html/ComplexHeatmap.html |
| Deeptools | Ramírez et al., 2016 | https://deeptools.readthedocs.io/en/develop/content/list_of_tools.html |
| SoftWoRx | Applied Precision, GE Healthcare | http://incelldownload.gehealthcare.com/bin/download_data/SoftWoRx/6.5.2/SoftWoRx.htm |
| Fiji | ImageJ | https://imagej.net/Using_Fiji |