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. 2019 Oct 10;221(5):841–850. doi: 10.1093/infdis/jiz522

Figure 2.

Figure 2.

Cell viability and major histocompatibility complex (MHC) expression of Bm1.11 cells infected with Chlamydia muridarum at different multiplicity of infection (MOI). (A) Bm1.11 cells were pretreated with 10 ng/mL interferon gamma for 6 hours and infected with C muridarum at different MOI. The inclusions after 18-hour infection were stained using anti-elementary body polyclonal antibodies. (B) Cell viability at different infection time points and MOI determined by trypan blue staining. The results represent the average of 3 replicate samples and are expressed as the means ± standard deviation. (C) Major histocompatibility complex class I and class II mean fluorescence intensity (MFI). Bm1.11 cells were pretreated with 10 ng/mL interferon gamma for 6 hours and infected with C muridarum at an MOI of 5 for 12, 14, 16, 18, or 20 hours. The cells were stained with anti-MHC I-fluorescein isothiocyanate and anti-MHC II-phycoerythrin. The cells not treated with interferon gamma and noninfected cells were set up as control. The data in C are representative of 2 similar experiments.