Figure 6.

The expansion of human dendritic cells (DCs) that coexpress CD141 and CD1c with differential human leukocyte antigen (HLA)-DR expression in human immune system (HIS) mice 1 day after AdPfCS/Adp24 vaccination. (A and B) Histograms (A) and line graphs (B), respectively, demonstrate the CD1c and CD141 geometric mean fluorescence intensities (MFI) within CD141⁺HLA-DR⁺CD11c⁺ DCs before (Day 0) and after (Day 1 and 4) AdPfCS/Adp24 vaccination. (C) Frequency of CD141⁺HLA-DR⁺CD11c⁺ DCs with or without CD1c expression that are CD86 positive in HIS mice 1 day after low dose versus high dose of AdPfCS/Adp24 vaccination. (D) Gating strategy for identifying HLA-DR^HIGH and HLA-DR^INTCD11c⁺ DCs (within peripheral lymph/human CD45⁺/Lin⁻ population). (E) Frequency of CD11c⁺ DC subsets that are single or double positive for CD141 and CD1c in blood, spleen, and lymph node (LN) of HIS mice 1 day after AdPfCS/Adp24 vaccination. Tissue samples, especially LN, were pooled from 7 individual HIS mice that were given injections of human hematopoietic stem cells (HSCs) from the same donor. The results are displayed by bar graphs expressing mean values and standard error (n = 3–7). The dashed line rectangle highlights the significant results for CD141⁺CD1c⁺ DCs. Statistical analyses were performed using analysis of variance followed by Dunnett’s test and differences were considered when P < .05. The vaccination experiments were repeated twice and data illustrate 1 representative experiment.