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FKBP25’s catalytic activity is required for homologous recombination (HR). (A) Western blot analysis of the U2OS DR-GFP homologous recombination reporter cells transfected with I-SceI and either an empty vector control, FKBP25(Wt), or FKBP25(Y198F) expression vectors. (B) Quantification of GFP U2OS DR-GFP reporter cells transfected as in A by flow cytometry. Error bars represent standard error of 3 independent transfections. *, P < 0.05.
