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. Author manuscript; available in PMC: 2020 Aug 31.
Published in final edited form as: Nat Microbiol. 2017 Mar 27;2:17037. doi: 10.1038/nmicrobiol.2017.37

Figure 5 |. cGAS restricts DENV replication.

Figure 5 |

Silencing of endogenous cGAS in primary human MDDCs. MDDCs from five independent donors were transfected with a specific cGAS siRNA or two different scramble siRNA as described in the Methods. At 48 h.p.i., cells were MOCK or DENV-2-infected (MOI = 0.5). a, Relative levels of cGAS mRNA were quantified by RT–qPCR at 4 h.p.i. b, Relative levels of IFN-α mRNA were quantified by RT–qPCR at 24 h.p.i. (data shows one representative donor out of five). Results are one representative of five independent experiments and are expressed as mean ± s.e.m. from three biological replicates (one-way ANOVA). c, Accumulation of DENV RNA was measured by RT–qPCR at 48 h.p.i. for the five donors tested. Results are expressed as mean from each of the five independent donors. Error bars represent mean ± s.e.m. d, Infectious DENV particles were quantified in the supernatant at 24 h.p.i. by plaque assay. Data show the mean ± s.e.m for five independent donors tested and showed a normal distribution.