Fig. 1.

Nutlin-3 stabilizes p53-MDM2 and HSP70-MDM2 protein-protein interactions in situ. a, c Proximity ligation assay of A375 cells treated with DMSO or 10 μM Nutlin-3 for 24 h. Green fluorescence spots suggest that both target proteins (p53-MDM2 or HSP70-MDM2) are within interacting range. The cells were counterstained with DAPI (blue). b, d The quantification of p53-MDM2 or HSP70-MDM2 interaction after incubation with DMSO and Nutlin-3. The results represent the mean ± SD of technical triplicates. Statistical analysis was performed by one-way ANOVA with a Bonferroni post hoc test, ** p < 0.01