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. 2020 Aug 31;25:41. doi: 10.1186/s11658-020-00233-w

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — Effects of CD276 depletion on cell cycle parameters. HCT116 p53-wt were transfected with siRNA towards CD276 (CD276si) for 48 h. a Detection of CD276 and β-actin on Western blot at molecular weights of 60 and 42 kDa, respectively, NTC – non-targeting control. b Flow cytometry analysis of cell cycle parameters. Distribution of G1 and G2 phases in HCT116 cells with normal and depleted CD276 protein level (CD276si) and after treatment with 20 μM Nutlin-3 or untreated for 48 h. Results represent the mean ± SD of technical triplicates, each with 10,000 counted cells. c Representative histograms of cell cycle after staining with propidium iodide