Table 1.
Morphological changes | SEM | TEM | ||
---|---|---|---|---|
Control | Buxus macowanii | Control | Buxus macowanii | |
Cell shape | Uniformly rod-shaped | swollen cells (CWS) | Uniformly rod-shaped | Cells are swollen and contracted |
Cell surface | Smooth cell surfaces | rough cell surface (RC) | Smooth cells surfaces | Rough cell surface (RC) |
Cytoplasmic membrane | Intact | aMembrane damage (DWC) and loss of intracellular contents (LCC) | Cytoplasm was homogenously electron dense | Partial loss of cytoplasmic electron density |
Cell wall | Intact | Damaged cell wall (DWC) (Perforations) | Intact | Cell wall distortion |
Cell division | Complete | Incomplete cell division (ICD) | Complete | Incomplete cell division (ICD) |
Cell viability | Viable | Cell death | Viable | Cell death |
aNo DNA extravasion carried out. However, the combination of the damage to the cell wall, perforations and other changes observed point to cytoplasmic membrane damage and loss of intracellular contents