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. 2020 Aug 21;13:8373–8382. doi: 10.2147/OTT.S248085

Figure 1.

Figure 1

Overexpression of ADAMTS8 inhibits proliferation, migration, invasion and induces apoptosis of BC cells. (A) Western blotting assays of ADAMTS8 protein expression in patients with BC tissues. T: BC tumor tissues; N: corresponding non-tumor tissues. (B) Expression of ADAMTS8 protein levels was examined in different BC cell lines with Western blotting. (C) Expression of ADAMTS8 protein was examined with Western blotting in MDA-MB-453 cells stably transfected with pEZ-M90-ADAMTS8 or pEZ-M90 plasmids. (D) CCK-8 assay showing that overexpression of ADAMTS8 inhibits proliferation of MDA-MB-453 cells. (E) Quantification of results from D. (F) Wound-healing assay showing that overexpression of ADAMTS8 suppresses migration of MDA-MB-453 cells. (G) Quantification of results from F. (H) Transwell assay showing that overexpression of ADAMTS8 suppresses invasion of MDA-MB-453 cells. (I, J) Quantification of results from H. (K) Flow cytometry showing that overexpression of ADAMTS8 induces apoptosis of MDA-MB-453 cells. (L) Quantification of results from (K). *P < 0.05, **P < 0.01.

Abbreviations: ADAMTS8, a disintegrin and metallopeptidase with thrombospondin motif 8; BC, breast cancer; CCK-8, cell counting kit-8.