Figure 3. Antibody neutralization of ΔPRRA mutant.

A) Schematic for SARS-CoV-2 ΔPRRA reporter virus expressing mNeonGreen (mNG) gene in place of ORF7 equivalent to previously described WT SARS-CoV-2 mNG virus²¹. B) Plaque reduction neutralization (PRNT₅₀) values as measured by changes to mNG expression. PRNT₅₀ values plotted as Log (1/serum dilution) with ΔPRRA on Y axis and WT-SARS-CoV-2. C-E) Representative curves from C) low, D) intermediate, and E) high neutralizing COVID-19 patient sera. F-H) Neutralization curves from mAB-1 (F), mAB-2 (G), and mAB-3 (H), N=3.