Table 2.

AUP values for example tag proteins and calculated fold-change decrease in AUP from standard design methods to the global min. AUP value.

	FLAG Tag	Strep Tag II	HA Tag	MYC Tag
Protein length	8	9	10	11
mRNA CDS lengtha	27	30	33	36
# Synonymous mRNAs	768	4,608	24,576	124,416
Standard methodsb, mean(std). AUP	0.86(7)	0.57(7)	0.67(9)	0.60(6)
Standard methodsb, min. AUP	0.68	0.45	0.47	0.45
Global Min. AUP	0.62	0.44	0.30	0.33
Mean standard / Global Min.	1.39	1.30	2.23	1.82
Min standard / Global Min.	1.10	1.02	1.57	1.36

^amRNA length = 3 × protein length + 3 (stop codon).

^bGENEWIZ, IDT, Twist.