Table 2.

AUP values for example tag proteins and calculated fold-change decrease in AUP from standard design methods to the global minimum AUP value.

	FLAG Tag	Strep Tag II	HA Tag	MYC Tag
Protein length	8	9	10	11
mRNA CDS lengtha	27	30	33	36
# Synonymous mRNAs	768	4,608	24,576	124,416
Standard methodsb, mean(std). AUP	0.86(7)	0.57(7)	0.67(9)	0.60(6)
Global Min. AUP	0.62	0.44	0.30	0.33
Mean standard / Global min.	1.39	1.30	2.23	1.82

^amRNA length = 3 × protein length + 3 (stop codon).

^bGENEWIZ, IDT, Twist.