Table 4.

Eterna OpenVaccine rounds, included mRNA design challenges, default folding engine, and default metrics provided to participants.

Title	Constructs	Metrics provided	Default folding engine	URL
[Lightning Round] Eterna takes on the Coronavirus	MEV, eGFP	ΔG(MFE), ΔG(ensemble)	ViennaRNA	https://eternagame.org/labs/9818634
[Round 2] Design of eGFP and epitope mRNA molecules	MEV, eGFP	ΔG(MFE), ΔG(ensemble)	ViennaRNA	https://eternagame.org/labs/9833861
[Round 3-extended] Lightning Round Design + Vote of Nanoluciferase	eGFP, Nluc	ΔG(MFE), ΔG(ensemble)	ViennaRNA	https://eternagame.org/labs/9874218
[Round 4] The 5’ UTR Design Lab	UTR design (not discussed here)	ΔG(MFE), ΔG(ensemble), # hairpins, longest stem, GC content	ViennaRNA	https://eternagame.org/labs/10027854
[Computational lab] Eterna vs. Computers, p(unp)	MEV, Nluc, eGFP, Spike RBD	ΔG(MFE), ΔG(ensemble), AUP	LinearFold/LinearPartition (ViennaRNA)	https://eternagame.org/labs/10097602
[Special Design Challenge] Humans vs. Computers take on the full spike protein	JEV+S	ΔG(MFE), ΔG(ensemble), # unpaired nucleotides	LinearFold/LinearPartition (ViennaRNA)	https://eternagame.org/labs/10126540
Round 7	S-2P	DegScore, DegScore Leaderboard, “branchiness”, Codon Usage, 3-way junctions, # hairpins, longest hairpin stem, GC content, energy of first 14 nts	LinearFold/LinearPartition (EternaFold)	https://eternagame.org/labs/10506533