Figure 2.

Efficacy of dynamic compression on BM-MSC and chondrocyte behavior. (a) Overview of study design. The constructs were motivated for 2 weeks with 1 h compression per day at 10% compressive strain (1 Hz, 23 h of rest period per day). (b) Immunofluorescence staining of aggrecan in chondrocyte and BM-MSC constructs under different conditions. Immunofluorescence staining of aggrecan (green) with nucleus (blue) in chondrocyte construct or BM-MSC construct. Scale bars = 50 μm. (c) Integrated fluorescence intensities of aggrecan in chondrocyte and BM-MSC constructs. The chondrocyte- and BM-MSC-seeded scaffolds under dynamic compression showed higher aggrecan staining than other conditions. Moreover, the aggrecan staining was found in SF-GCH constructs than in SF constructs under dynamic compression. Adopted from Sawatjui et al. and reprinted with permission of John Wiley and Sons.⁶¹