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. 2020 Aug 27;12:1758835920937890. doi: 10.1177/1758835920937890

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© The Author(s), 2020

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 1. — Robo1 was highly expressed in HCC tissues and cells. (A) the expression of Robo1 in 18 cases of HCC tissue samples determined by RT-qPCR, normalized to GAPDH; the number in the graph represented the sample number; (B) the expression of Robo1 in 18 cases of HCC tissues, 18 cases of paracancerous tissues and 12 cases of normal liver tissues examined by RT-qPCR; (C) the protein expression of Robo1 in HCC, paracancerous and normal liver tissues detected by Western blot analysis. The value of each sample point in the statistical graph = gray value of Robo1 protein band in samples/gray value of GAPDH protein band in samples/normalization value. (^*p < 0.05, ^**p < 0.01, ^***p < 0.001); (D) the expression of Robo1 in HCC tissues examined by immunohistochemistry, and immunofluorescence staining (left, 200×; right, 400×); (E) the correlation between survival rate of HCC patients and expression of Robo1 analyzed by Kaplan–Meier method; (F) the correlation between recurrence rate of HCC patients and expression of Robo1 analyzed by Kaplan–Meier method; (G) the expression of Robo1 in HCC and normal liver cell lines; (H) the expression of Robo1 in HCC and normal liver cell lines determined by Western blot analysis; n = 3. Data were analyzed using one-way analysis of variance.

GAPDH, glyceraldehyde-3-phosphate dehydrogenase; HCC, hepatocellular carcinoma; RT-qPCR, reverse transcription quantitative polymerase chain reaction.