. Author manuscript; available in PMC: 2020 Aug 31.

Published in final edited form as: ACS Nano. 2020 Feb 20;14(3):3703–3717. doi: 10.1021/acsnano.0c00962

Table 2.

Sequences for sgRNAs Targeting Human LC3B, ATG16L, and BECN1 Genes and the Primers for the Surveyor Assay

LC3B sgRNA	TTCAAGCAGCGCCGCACCTT
ATG16L1 sgRNA	CAATTTAGTCCCGGACATGA
BECN1 sgRNA	ATTTATTGAAACTCCTCGCC
LC3B KO Surveyor primer forward	CGCCAGAGTCGGATTCGCCG
LC3B KO Surveyor primer reverse	CGGGGTGATTCAGCAGGCCC
ATG16L KO Surveyor primer forward	GGCTTTGTGAACATGTTTCTG
ATG16L KO Surveyor primer reverse	GGGCCTCAATCTGCCTGGTC
BECN1 KO Surveyor primer forward	CCTGACTGCTAAGTGTTGAG
BECN1 KO Surveyor primer reverse	CATCTCTATCACCTGGCTC