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. 2020 Aug 25;12:7837–7846. doi: 10.2147/CMAR.S248318

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© 2020 Sun et al.

This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).

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Verification of NCAPG knockdown in AGS and HGC-27 cells, and the effect of stable NCAPG knockdown on cell growth and cell cycle distribution. The Western blotting results (A) Indicate NCAPG was efficiently knockdown by shRNA treatment. (B) Stable NCAPG knockdown resulted in remarkedly decreased colony formation. (C) Stable NCAPG knockdown resulted in a sustained accumulation of cells in the G0/G1 phase. Cellular distribution (as percentages) in different phases of the cell cycle (G0/G1, S, and G2/M) is presented. (D) Stable NCAPG knockdown resulted in dysregulated expressions of G0/G1-related molecules. A typical result from three independent experiments is shown.

Abbreviations: WT, non-transduced AGS and HGC-27 cells; Scrambled con, scrambled control AGS and HGC-27 cells; NCAPG shRNA, NCAPG knockdown AGS and HGC-27 cells.