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. 2020 Aug 31;21:596. doi: 10.1186/s12864-020-07020-z

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Circadian behaviour of nocturnal and diurnal selection flies. a. Boxplots of circadian periods under free-run conditions. Statistical differences were tested by a TukeyHSD test, with * signifying p < 0.05. b. Acrophase angles of the free-run activity for N (black circles, n = 255), D (red circles, n = 159) and C (blue triangles, n = 65) populations. The free-run phases of the three populations did not differ (F_2,476:1.91, p = 0.149, NS). Lines represent mean vectors ±95% confidence interval (CI). One hour corresponds to an angle of 15°. C. Phase delay angles are shown for N (black circles, n = 153), D (red circles, n = 155) and C (blue triangles, n = 56) populations. Differences are not significant (F_2,361 = 1.47, p = 0.23, NS). Lines represent mean vectors ±95% CI