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. 2020 Aug 24;12:7753–7760. doi: 10.2147/CMAR.S249296

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© 2020 Wang and Xu.

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PART1 binds to miR-4516. (A) The predicted target site between PART1 and miR-4516. (B) Relative expression of miR-4516 was detected using qRT-PCR after overexpression of miR-4516 or not. Fold change was normalized to 18S. (C) The interaction between PART1 and miR-4516 was measured by luciferase reporter assay in MCF-7 cells. MCF-7 cells were co-transfected with the WT reporter plasmid (or the corresponding mutant reporter) and the miR-4516. (D) Relative expression of miR-4516 was detected using qRT-PCR after knockdown of PART1. Fold change was normalized to 18S. (E) Relative expression of miR-4516 was detected using qRT-PCR in breast cancer tissues compared with adjacent healthy tissues. Fold change was normalized to 18S. *P<0.05. All experiments were repeated three times.