Figure 2.

Influence of circ_0000735 silencing on proliferation, migration, invasion, and glycolysis of NSCLC cells. (A and B) The abundant of circ_0000735 in the cytoplasm and nuclear of A549 and H1299 cells was analyzed by RT-qPCR assay, GAPDH and U6 were used as positive controls, respectively. (C–K) A549 and H1299 cells were administrated with si-circ_0000735 or si-NC. (C) The interference efficiency of si-circ_0000735 was checked by RT-qPCR assay. (D and E) Effect of circ_0000735 silencing on proliferation of A549 and H1299 cells was measured with CCK-8 and colony-forming assays. (F and G) Transwell migration and invasion assays were implemented to examine the migration and invasion ability of A549 and H1299 cells. (H and I) Extracellular acidification rate (ECAR) of A549 and H1299 cells was shown. (J and K) Lactate production was detected in A549 and H1299 cells by indicated kit. *P<0.05.

Abbreviations: Glc, glucose; O, oligomycin; 2-DG, 2-deoxy-D-glucose.