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. 2020 Aug 24;13:8427–8439. doi: 10.2147/OTT.S253474

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© 2020 Huang et al.

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miR-940 was a direct target of circ_0000735. (A) Binding domain between miR-940 and circ_0000735, as well as matched mutated nucleotide sites were shown. (B and C) Dual-luciferase reporter assay was employed to detect the luciferase activity in A549 and H1299 cells co-transfected with WT-circ_0000735 or MUT-circ_0000735 and miR-940 mimic or miR-NC, respectively. (D and E) RT-qPCR assay was performed to evaluate the expression level of miR-940 in A549 and H1299 cells infected with si-circ_0000735, circ_0000735, si-NC, or vector. *P<0.05.