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. 2020 Aug 24;13:8427–8439. doi: 10.2147/OTT.S253474

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© 2020 Huang et al.

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Overexpression of miR-940 impeded proliferation, migration, invasion, and glycolysis of NSCLC cells. (A and B) The expression level of miR-940 was tested with RT-qPCR in NSCLC tissue and adjacent normal tissue, as well as in 16HBE, A549, and H1299 cells. (C) RT-qPCR was performed to confirm the overexpression efficiency of miR-940 in A549 and H1299 cells. (D and E) CCK-8 and colony-forming assays were applied to evaluate cell viability of A549 and H1299 cells transfected with miR-940 or miR-NC. (F and G) The migration and invasion abilities of A549 and H1299 cells were estimated by transwell assay after transfection with miR-940 or miR-NC. (H) The extracellular acidification rate curves were plotted using glucose, oligomycin, and 2-deoxy-D-glucose at specific times. (I) Lactate production level was detected in A549 and H1299 cells after being transfected with miR-940 or miR-NC. *P<0.05.