Figure 7.

Upregulation of BMPER reversed si-circ_0000735-induced the effects on proliferation, migration, invasion, and glycolysis in NSCLC cells. (A) The protein expression level of BMPER was measured by Western blot assay in A549 and H1299 cells infected with vector or BMPER. (B–H) A549 and H1299 cells were infected with si-NC, si-circ_0000735, si-circ_0000735+vector, or si-circ_0000735+BMPER. (B) Western blot assay was performed for examining protein expression of BMPER in A549 and H1299 cells posttransfection. (C and D) The cell proliferation of A549 and H1299 cells was assessed by CCK-8 and colony-forming assays. (E and F) Cell migration and invasion capabilities were examined in transfected A549 and H1299 cells using transwell analysis. (G) Extracellular acid ratio of cells was measured in A549 and H1299 cells after transfection. (H) The lactate production of transfected A549 and H1299 cells were shown. *P<0.05.