Table 2.
Limits of detection of the variplex™ RT-LAMP and LightMix® RT-PCR conducted on RNA eluates from SARS-CoV-2 virus stock dilutions.
| Virus concentration |
Variplex™ M |
LightMix® E |
|
|---|---|---|---|
| TCID50/mL | TCID50/reaction | Positive replicates M gene, mean threshold time [min] (SD) | Positive replicates E gene, mean Ct (SD) |
| 100 | 3 | 6/6, 8.5 (1.25) | 6/6, 25.4 (0.5) |
| 10 | 0.3 | 6/6, 11.5 (1.75) | 6/6, 28.8 (0.5) |
| 1 | 0.03 | 6/6, 22.25 (6.25) | 6/6, 33.3 (1) |
| 0.1 | 0.003 | 2/6, 36 | 6/6, 36.1 (1.5) |
| 0.01 | 0.0003 | – | 2/6, 37.1 |
| 0.001 | 0.00003 | – | – |