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. 2019 Aug 14;8(11):e1647760. doi: 10.1080/2162402X.2019.1647760

Figure 4.

Figure 4.

Wild type (wt), Annexin A1 (AnxA1−/-), Formyl peptide receptor 1 (Fpr1−/-) and mitochondrial methionyl-tRNA formyltransferase (Mtfmt−/-) deficient murine MCA205 fibrosarcoma (a,c) or TC-1 non small cell lung carcinoma (b,d) cells were treated with phosphate-buffered saline (PBS), 2 μM mitoxantrone (MTX), 25 or 20 µM doxorubicin (DOXO) for 18 and 24 h, respectively. Cells were subsequently assessed by cytofluorometric immunodetection of calreticulin (CALR) exposure. Results from one representative experiment out of three independent ones yielding similar results are reported in (b,d). Data are represented as mean ± SD. Representative cytofluorometric profiles for one sample out of three independent ones belonging to at least 3 independent experiments are reported in (a,c). **** p < 0.0001 (unpaired Student’s t test), as compared to PBS-treated cells. #### p < 0.0001 (unpaired Student’s t test), as compared to CALR exposure in WT cells. Immunobloting (e) and quantification ((f), mean ± SEM, n = 2) for CALR in MCA205 and TC-1 WT or AnxA1−/- cells. * p < 0.05, ** p < 0.01 (unpaired Student’s t test), as compared to WT cells. PI, Propidium iodide.