Figure 7.
Enhanced inflammatory cytokine production by PBMCs from SCFP-treated calves compared with controls. PBMCs were isolated from peripheral blood on day 19 of the study (day 0 prior to BRSV infection) and cryopreserved. PBMCs were plated at 1 × 106 cells/well in 24-well plates and stimulated for 72 h with 1 μg/mL LPS, 10 μg/mL Pam3CSK4, or a mixture of 50 μg/mL Poly(I:C) with 10 μg/mL imiquimod. Mock wells were treated with media only. After 72 h, cell culture supernatants were collected and analyzed by commercial ELISA kit for concentrations of IL-6, TNFα, or IL-1β; n =10 control calves; n = 12 SCFP calves.