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. 2020 Aug 31;28(22):115713. doi: 10.1016/j.bmc.2020.115713

Fig. 6.

Fig. 6

Effect of increasing concentrations of 1-TP (2MR04) on DENV polymerase activity. An elongation complex was formed of DENV NS5 and a primer/template combination corresponding to the 5’ end of DENV2 genome and the 3’ end of the antigenome. Primer elongation reactions were done in presence of all NTPs or in absence of GTP to test the incorporation of 1-TP as a GTP analogue. Reaction mixtures were analyzed by denaturating PAGE and substrate (P10) and product bands were visualized by autoradiography.