Table 4.
RA (%) of bacterial families and fungal species in corn silages untreated or treated with an additive containing sodium benzoate, potassium sorbate, and sodium nitrite subjected or not to air stress ensiled for 63 d, as analyzed by the sequencing of the V4-V5 region of the 16S rRNA, for bacteria, and ITS1, for fungi, using the Illumina MiSeq platform
Bacterial families | Fungal species | ||||||
---|---|---|---|---|---|---|---|
Item1 | Lactobacillaceae | Enterobacteriaceae | Leuconostocaceae | Acetobacteraceae | C. tropicalis | C. humilis | P. kudriavzevii |
Air stress × additive | |||||||
NS | |||||||
CTRL | 62.03c | 7.30a | 10.26a | 0.02 | 77.01 | 0.08b | 1.02b |
SF2 | 79.76ab | 1.87b | 2.58b | 0.00 | 49.82 | 0.05b | 0.55b |
AS2 | |||||||
CTRL | 84.28a | 4.02ab | 2.95b | 1.36 | 21.91 | 0.04b | 71.51a |
SF2 | 63.56bc | 4.69ab | 4.56b | 8.99 | 0.49 | 98.09a | 0.00b |
Additive means | |||||||
CTRL | 73.16 | 5.66 | 6.61 | 0.69 | 49.46a | 0.06 | 36.27 |
SF2 | 71.66 | 3.28 | 3.57 | 4.50 | 25.16b | 49.07 | 0.27 |
Air stress means | |||||||
NS | 70.90 | 4.58 | 6.42 | 0.01 | 63.42a | 0.07 | 0.78 |
AS | 73.92 | 4.36 | 3.75 | 5.17 | 11.20b | 49.07 | 35.76 |
SEM | 5.68 | 1.14 | 1.10 | 2.73 | 8.25 | 0.33 | 5.76 |
Significance | ----------------------------------------------------------- P–value3 ----------------------------------------------------------------- | ||||||
Air stress | 0.60 | 0.85 | 0.03 | 0.08 | <0.01 | <0.01 | <0.01 |
Additive | 0.80 | 0.06 | 0.02 | 0.19 | 0.01 | <0.01 | <0.01 |
Air stress × additive | <0.01 | 0.02 | <0.01 | 0.19 | 0.73 | <0.01 | <0.01 |
1NS, no air stress; CTRL, control; SF2, Safesil at 2 L/t of fresh forage weight.
2AS, 2 h/wk air stress.
3Means in columns within sections with unlike superscripts differ (P < 0.05).