Table 1. Detection of T.cruzi DNA in infants born to seropositive mothers using Tc LAMP and qPCR.
| Cases ID | Microscopy observation | Serological Findings | Gold standard Final Diagnosis | Treatment status | Molecular Analysis | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Mtnity. Result (Age in days) | H. Ctr. Result (Age in days) | ELISA | HAI ¤ | Final Result* (Age in days) | Type of Sample (Age in days) | LAMP Result | SatDNA qPCR Result | Parasitic Load** (p.e/mL) | |||
| cCD1 | Nav | Neg (63) | 0.759 Ɛ | 64 | Reactive (336) | cCD | Not treated | VB (336) | Pos | Pos | 5.27 |
| cCD2 | Nav | Pos (87) | 0.015 ß | NR | Non Reactive-treated (600) | cCD | Completed | VB (87) | Pos | Pos | 3683.8 |
| cCD3 | Nav | Neg (36) | 2.985 ß | 256 | Reactive (368) | cCD | Not treated | VB (36) | Pos | Pos | 356.19 |
| cCD4 | Nav | Pos (10) | 0.036 ¥ | NR | Non Reactive-treated (275) | cCD | Completed | VB (10) | Pos | Pos | 640.98 |
| cCD5 | Nav | Pos (49) | 2.501 ß | 256 | Reactive (322) | cCD | Not treated # | VB (49) | Pos | Pos | 37.47 |
| cCD6 | Nav | Pos (8) | 2.624 § | 256 | Reactive (303) | cCD | Not treated ## | VB (303) | Pos | Pos | 3063.47 |
| cCD7 | Neg (1) | Neg (41) | 2.531 ß | 128 | Reactive (427) | cCD | Not treated | VB (41) | Pos | Pos | 53.35 |
| cCD8 | NAv | Neg (45) | 1.418 ¥ | 256 | Reactive (331) | cCD | Not treated | VB (45) | Pos | Pos | 81.96 |
| cCD9 | Pos (1) | Neg (44) | 0.059 ¥ | NR | Non Reactive-treated (324) | cCD | Completed | VB (44) | Pos | Pos | 12.96 |
| cCD10 | Pos (2) | NA | Nav | NR | Non Reactive-treated (Nav) | cCD | Completed | DBS (2) | Pos | Pos | NA |
| cCD11 | Pos (2) | NA | Nav | NR | Non Reactive-treated (Nav) | cCD | Completed | DBS (2) | Pos | Pos | NA |
| cCD12 | Pos (2) | NA | Nav | NR | Non Reactive-treated (Nav) | cCD | Completed | DBS (2) | Pos | Pos | NA |
| cCD13 | Pos (2) | NA | Nav | NR | Non Reactive-treated (Nav) | cCD | Completed | DBS (2) | Pos | Pos | NA |
| cCD14 | Nav | Neg (10) | 0.016 § | NR | Non Reactive (435) | Non Infected | NA | VB (435) | Neg | Neg | NA |
| cCD15 | Nav | Neg (104) | 0.036 ¥ | NR | Non Reactive (306) | Non Infected | NA | VB (104) | Neg | Neg | NA |
| cCD16 | Nav | Neg (112) | 0.014 ß | NR | Non Reactive (312) | Non Infected | NA | VB (312) | Neg | Neg | NA |
| cCD17 | Nav | Neg (64) | 0.015 ß | NR | Non Reactive (350) | Non Infected | NA | VB (64) | Neg | Neg | NA |
| cCD18 | Nav | Neg (40) | 0.016 ß | NR | Non Reactive (319) | Non Infected | NA | VB (40) | Neg | Neg | NA |
| cCD19 | Nav | Neg (30) | 0.039 ¥ | NR | Non Reactive (304) | Non Infected | NA | VB (304) | Neg | Neg | NA |
| cCD20 | Neg (1) | Neg (31) | 0.038 ¥ | NR | Non Reactive (304) | Non Infected | NA | VB (31) | Neg | Neg | NA |
| cCD21 | Neg (2) | Neg (33) | 0.015 ß | NR | Non Reactive (306) | Non Infected | NA | VB (2) | Neg | Neg | NA |
| cCD22 | Neg (1) | Neg (62) | 0.022 ¥ | NR | Non Reactive (321) | Non Infected | NA | VB (321) | Neg | Neg | NA |
| cCD23 | Nav | Neg (122) | 0.018 ß | NR | Non Reactive (326) | Non Infected | NA | VB (326) | Neg | Neg | NA |
| cCD24 | Nav | Neg (60) | 0.036 ¥ | NR | Non Reactive (304) | Non Infected | NA | VB (304) | Neg | Neg | NA |
| cCD25 | Nav | Neg (28) | 0.014 ß | NR | Non Reactive (307) | Non Infected | NA | VB (28) | Neg | Neg | NA |
| cCD26 | Neg (2) | NA | 0.014 ß | NR | Non Reactive (305) | Non Infected | NA | VB (2) | Neg | Neg | NA |
| cCD27 | Neg (2) | NA | 0.012 ß | NR | Non Reactive (329) | Non Infected | NA | VB (2) | Neg | Neg | NA |
| cCD28 | Neg (2) | NA | 0.014 ß | NR | Non Reactive (346) | Non Infected | NA | VB (2) | Neg | Neg | NA |
| cCD29 | Neg (2) | NA | 0.017 ß | NR | Non Reactive (356) | Non Infected | NA | VB (2) | Neg | Neg | NA |
| cCD30 | Neg (1) | NA | 0.071¥ | NR | Non Reactive (316) | Non Infected | NA | VB (1) | Neg | Neg | NA |
Ɛ cut-off: 0.152;
§ cut-off: 0.318;
ß cut-off: 0.316;
¥ cut-off: 0.322;
¤ cut-off: 1
Mtnity: maternity service. H.Ctr: health center. M: male; F: female; MD: missing data; VB: venous blood containing 25% of DNAgard stabilizer; DBS: dried blood spot; Nav: sample not available; NA: not applicable; Pos: positive; Neg: negative; p.e/mL: parasite equivalents/milliliter.
* The criterion for serological reactivity was based on at least two positive serological tests.
** For PCR quantification, the standard curve consisted of DNA obtained from seronegative human blood spiked with known amounts of CL Brener cultured parasites (TcVI).
# The patient did not complete treatment;
## Comorbidities: bronchiolitis and nosocomial infection.