Table 1. IncQ plasmids introduced into N. meningitidis strains which results were shown in this study.
Plasmid | Relative properties | Antibiotic selection marker | References |
---|---|---|---|
pHT128 | Derivative of pGSS33, an IncQ broad-host -range vector | Tet, Cml | [33] |
pHT1212 | Derivative of pHT128 carrying a lacIq-Ptac-MmPylRS[Y306A/Y384F] and two Plpp-tRNApyl genes | Tet, Cml | This study |
pHT1262 | Derivative of pHT128 carrying a lacIq-MmPylRS([A302T/N346T/C348T/W417C]) and two Plpp-tRNApyl genes | Tet, Cml | This study |
pHT936 | Derivative of pHT128 carrying lacIq-Ptac-gst+ genes | Cml | This study |
pHT1355 | Derivative of pHT1212 carrying a Ptac-gst E51amb gene | Cml | This study |
pHT1356 | Derivative of pHT1212 carrying a Ptac-gst F52amb gene | Cml | This study |
pHT1357 | Derivative of pHT1262 carrying a Ptac-gst E51amb gene | Cml | This study |
pHT1358 | Derivative of pHT1262 carrying a Ptac-gst F52amb gene | Cml | This study |
pHT1263 | Derivative of pHT1262 carrying a pamA+-His6 gene | Cml | This study |
pHT1270 | Derivative of pHT1262 carrying a pamA K208amb-His6 gene | Cml | This study |
pHT1274 | Derivative of pHT1262 carrying a pamA K278amb-His6 gene | Cml | This study |
pHT1276 | Derivative of pHT1262 carrying a pamA K309amb-His6 gene | Cml | This study |
pHT1279 | Derivative of pHT1262 carrying a pamA K341amb-His6 gene | Cml | This study |
pHT1282 | Derivative of pHT1262 carrying a pamA K382amb-His6 gene | Cml | This study |
pHT1284 | Derivative of pHT1262 carrying a pamA K395amb-His6 gene | Cml | This study |
pHT1388 | Derivative of pHT1262 carrying a pamA K278amb-StrepTag2-His6 gene | Cml | This study |
Tet and Cml stand for tetracycline and chloramphenicol resistance marker, respectively.