Skip to main content
. 2020 Aug 31;15(8):e0237883. doi: 10.1371/journal.pone.0237883

Table 1. IncQ plasmids introduced into N. meningitidis strains which results were shown in this study.

Plasmid Relative properties Antibiotic selection marker References
pHT128 Derivative of pGSS33, an IncQ broad-host -range vector Tet, Cml [33]
pHT1212 Derivative of pHT128 carrying a lacIq-Ptac-MmPylRS[Y306A/Y384F] and two Plpp-tRNApyl genes Tet, Cml This study
pHT1262 Derivative of pHT128 carrying a lacIq-MmPylRS([A302T/N346T/C348T/W417C]) and two Plpp-tRNApyl genes Tet, Cml This study
pHT936 Derivative of pHT128 carrying lacIq-Ptac-gst+ genes Cml This study
pHT1355 Derivative of pHT1212 carrying a Ptac-gst E51amb gene Cml This study
pHT1356 Derivative of pHT1212 carrying a Ptac-gst F52amb gene Cml This study
pHT1357 Derivative of pHT1262 carrying a Ptac-gst E51amb gene Cml This study
pHT1358 Derivative of pHT1262 carrying a Ptac-gst F52amb gene Cml This study
pHT1263 Derivative of pHT1262 carrying a pamA+-His6 gene Cml This study
pHT1270 Derivative of pHT1262 carrying a pamA K208amb-His6 gene Cml This study
pHT1274 Derivative of pHT1262 carrying a pamA K278amb-His6 gene Cml This study
pHT1276 Derivative of pHT1262 carrying a pamA K309amb-His6 gene Cml This study
pHT1279 Derivative of pHT1262 carrying a pamA K341amb-His6 gene Cml This study
pHT1282 Derivative of pHT1262 carrying a pamA K382amb-His6 gene Cml This study
pHT1284 Derivative of pHT1262 carrying a pamA K395amb-His6 gene Cml This study
pHT1388 Derivative of pHT1262 carrying a pamA K278amb-StrepTag2-His6 gene Cml This study

Tet and Cml stand for tetracycline and chloramphenicol resistance marker, respectively.