Table 2.
Serological (ELISA, IFAT, SNAP tests) and PCR (conjunctival swabs and bone marrow aspirates) follow-up of dogs.
| Days | Group 1 Untreated control |
Group 2 Frontline Tri-Act treated | |
|---|---|---|---|
| Positive n / N (%), Dog ID, Technique |
|||
| Serology | PCR | Serology and PCR | |
| Day 112 | 1/20 (5 %) | 0/20 (0 %) | 0 / 20 (0 %) |
| 6 | PCR conjunctival swabs | ELISA, PCR conjunctival swabs | |
| ELISA | |||
| Day 140 | 2/20 (10 %) | 2/20 (10 %) | 0 / 20 (0 %) |
| 6, 23 | 6, 37 | SNAP, PCR conjunctival swabs | |
| SNAP | PCR conjunctival swabs | ||
| Day 168 | 3/20 (15 %) | 4/20 (10 %) | 0 / 20 (0 %) |
| 6, 23, 33 | 6, 23, 33, 37 | SNAP, PCR conjunctival swabs | |
| SNAP | PCR conjunctival swabs | ||
| Day 252 | 4/20 (10 %) | 4/20 (10 %) | 0 / 20 (0 %) |
| 6, 23, 33, 37 | 6, 23, 33, 37 | ELISA, SNAP, IFAT, PCR conjunctival swabs & bone marrows | |
| ELISA, SNAP, IFAT | PCR conjunctival swabs & bone marrows | ||
| Day 350 | 6 / 20 (20 %) | 7 / 20 (20 %) | 0 / 20 (0 %) |
| 3, 6, 23, 30, 33, 37 | 3, 6, 15, 23, 30, 33, 37 | ELISA, SNAP, IFAT, PCR conjunctival swabs & bone marrows | |
| ELISA, SNAP, IFAT | PCR conjunctival swabs & bone marrows | ||
| p = 0.02 (Fischer’s exact test compared to treated group) | p = 0.0083(Fischer’s exact test compared to treated group) | ||
Group 1: Dogs were treated with sterile water, intended as a "sham-treatment" to maintain blinding, as a topical spot-on on Days 0, 28, 56, 84, 112, 140 and 168 Group 2: Dogs were treated with Frontline Tri-Act® as a topical spot-on on Days 0, 28, 56, 84, 112, 140 and 168.