Fig. 6. Effects of C2I treatment on changes in tau phosphorylation in WT mice 1 month after repeated concussions.

WT mice were implanted with Alzet minipumps delivering vehicle [400 mg/ml; (2-hydroxypropyl)-β-cyclodextrin] or C2I (0.3 mg kg⁻¹ day⁻¹) 1 day before 10 days of repeated concussions. Pumps were withdrawn 4 days after the last day of concussion, and the animals were sacrificed 4 weeks later. (A, C, and E) Changes in tau phosphorylation in cortex, corpus callosum, and optic tract. Brains were fixed and processed for IHC with p-tau Thr²³¹ antibodies. Scale bars, 20 μm. (B, D, and F) Quantification of images similar to those shown. n = 8 for veh sham and veh rmTBI; n = 7 for C2I sham; and n = 9 for C2I rmTBI. *P < 0.05, **P < 0.01, and ***P < 0.001. One-way ANOVA followed by Bonferroni’s test. Data represent means ± SEM.