Table 5. Details of Sample Replicates and Statistical Tests Used in Figures.
| figure | sub figure | name | statistical analysis | donors | biological replicates | technical replicates |
|---|---|---|---|---|---|---|
| 1 | B | Interferometry 0.8 vs 1.8 mg/mL | Mann–Whitney | – | 24 | 5 |
| C | Interferometry Freq-amplitude | – | – | 3–5 | 5 | |
| D | Interferometry Plateform-gel | Mann–Whitney | – | 24 | 5 | |
| E | Interferometry Vol-amplitude | – | – | 5 | 5 | |
| F | Interferometry Freq-amplitude | – | – | 5 | 5 | |
| G | RT-PCR day9 | One-way ANOVA/Tukey | 2 | 4 | 3 | |
| H | temporal gene analysis of phenotype | Mann–Whitney | 3 | 4 | 3 | |
| 2 | A | protein array heatmap | - | 1 | 4 | - |
| B | PCR-ion channel | One-way ANOVA/Tukey | 2 | 4 | 3 | |
| C | PCR-BMP2 | One-way ANOVA/Tukey | 2 | 4 | 3 | |
| D | PCR-Col1A | One-way ANOVA/Tukey | 2 | 4 | 3 | |
| E | PCR-ROCK inhibition | One-way ANOVA/Tukey | 1 | 4 | 3 | |
| 3 | A-D | metabolomics | – | 1 | 4 | - |
| 4 | A-C, F | metabolomics | – | 1 | 4 | - |
| B | ROS (DCF-DA) | One-way ANOVA/Tukey | 3 | 3 | 1 | |
| E | PCR, ROS with NAC | One-way ANOVA/Tukey | 1 | 4 | 3 | |
| 5 | A | PCR inflammation | Mann–Whitney | 1 | 4 | 3 |
| B | PCR, Effect of dependent pathway | Mann–Whitney | 1 | 4 | 3 | |
| C | PCR-temporal gene study | One-way ANOVA/Tukey | 3 | 4 | 3 | |
| D | ELISA-1β | One-way ANOVA/Tukey | 1 | 4 | 1 | |
| C | Contraction | Two-way ANOVA/Tukey | 1 | 4 | 1 | |
| D | Interferometry | – | – | 3–6 | 5 | |
| E | Interferometry Freq-amp | – | – | 3 | 5 | |
| F | direction | – | 1 | 1 | 10 | |
| G | velocity | Mann–Whitney | 1 | 1 | 10 | |
| H | cryosection and staining | – | 1 | 2 | – | |
| 7 | A,B | PCR, temporal study | One-way ANOVA/Tukey | 2 | 4 | 3 |
| D | PCR-memory test | Mann–Whitney | 1 | 4 | 3 | |
| S1 | A | rheology | Mann–Whitney | – | 3 | 1 |
| B | hydrogel contraction | Two-way ANOVA/Tukey | 1 | 4 | 1 | |
| C | AlamarBlue | One-way ANOVA/Tukey | 1 | 3–5 | 1 | |
| S2 | – | PCR-non responding pt-NFkB | One-way ANOVA/Tukey | 1 | 4 | 3 |
| S3 | – | -Paired-t test (same gel) | 1 | 4 | 1 | |
| -One-way ANOVA/Tukey |