Figure 3.

RNA-LPX-vaccinated mice premedicated with Dexa exhibit low CD4⁺ and CD8⁺ blood T-cell numbers and elevated Treg fractions. (a), CD4⁺, CD8⁺ or CD45⁺ cells per µL peripheral blood 5 d after the first treatment of BALB/c mice (n = 9, upper panel) with gp70 RNA-LPX or C57BL/6 mice (n = 5, lower panel) with Reps1 + Adpgk RNA-LPX and Dexa co-medication (see Figure 1(a)) as quantified by flow cytometry. (b), CD4⁺ CD25⁺ FoxP3⁺ Tregs per µL peripheral blood 5 d after the first vaccination (left) and fraction of Tregs within the CD4⁺ T cell population after two vaccinations (right) of BALB/c mice (upper panel, n = 9, left; n = 5, right) treated with gp70 RNA-LPX, or C57BL/6 mice (lower panel, n = 5) treated with Reps1 + Adpgk RNA-LPX and Dexa co-medication. Mean (line) and individual values (symbols) are shown. The dotted line represents the mean of the control group.