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. 2020 Aug 20;9(1):1803530. doi: 10.1080/2162402X.2020.1803530

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© 2020 The Author(s). Published with license by Taylor & Francis Group, LLC.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Enhanced immunogenicity of cancer stem-like cells CD133⁺ HM-1 cells. (a) Tumor growth curve of bulk and CD133⁺ HM-1 cells showed similar growth pattern (left). However, CD133⁺ HM-1 cells expressing chicken ovalbumin (CD133⁺ HM-1-ova) were rejected. Bulk HM-1 cells expressing ovalbumin grew slowly but could still form the tumors (p < .0001, right). (b) Suppression of CD133⁺ HM-1-ova tumor growth was abolished through depleting CD8⁺ and CD4⁺ T lymphocytes by TIB210 (anti-CD8) and GK1.5 (anti-CD4) neutralizing antibodies (p < .001), suggesting rejection of CD133⁺ HM-1-ova tumor involved CD8⁺ and CD4⁺ T cell immune response. (c) Mice were either not immunized (naïve), immunized with bulk HM-1 or CD133⁺ HM-1 cells. The re-stimulated antigens were either nothing (control), bulk or CD133⁺ HM-1 cells. Splenocytes from mice immunized with CD133⁺ HM-1 cells are associated with more CD8⁺ IFN-γ⁺ T lymphocyte by the same stimulating cells (p < .001, Bulk bulk vs CD133⁺ CD133⁺; p < .05, Bulk bulk vs CD133⁺ bulk, the former is cells for immunization, the latter is cells for re-stimulation in assay). Vaccination with bulk cells induced less activated CD8⁺ T lymphocytes. (d) CFSE-pulsed bulk and CD133⁺ HM-1 cells were incubated with syngeneic CD11c⁺ cells for 6 hours. Phagocytosis was analyzed by flow cytometry for CFSE-stained CD11c⁺ cells. Increased phagocytosis of CD11c⁺ cells was observed on both live and irradiated CD133⁺ HM-1 cells (p < .0001 on both). ^#p < .05, *p < .01, **p < .001, ***p < .0001, NS: not significant.