Figure 1.
Multiple ARG2 peptides are recognized by PBMCs from healthy donors. (a) IFNγ ELISPOT screening of responses against overlapping 20-mer ARG2 peptides from six healthy donors. 4–4.5 × 105 cells were plated per well, and peptide and control stimulation were performed in duplicate or triplicate. Specific spot counts (peptide-specific IFNγ-secreting cells) are given as the difference in number of IFNγ spots between averages of the wells stimulated with peptide and control wells. (b) IFNγ ELISPOT responses from the screening toward the peptides here covering the signal peptide region of ARG2 (left; ARG2-0, ARG2-1, ARG2-2) or the peptides located in the region corresponding to the most immunogenic region of the ARG1 sequence (right; ARG2-17, ARG2-18, ARG2-19, ARG2-20 og ARG2-21). (c) Alignment of ARG1 and ARG2 amino acid sequence around the ARG2-1 sequence. The ARG2-1 sequence is highlighted and marked in red.