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. 2020 Jun 23;9(1):1782575. doi: 10.1080/2162402X.2020.1782575

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© 2020 The Author(s). Published with license by Taylor & Francis Group, LLC.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Monocyte secretion of free inflammatory cytokines before and after RUX therapy or in vitro RUX treatment. Concentrations of crucial cytokines (IL1β, TNF-α, IL6, IL10) in the supernatants of immunomagnetically isolated monocytes from healthy donors (HD; n = 20) and MF patients at baseline ((MF baseline T0; n = 20) and after 6 months of RUX therapy (MF RUX (T6 m); n = 20) in vitro cultured for 4/24 hours in the presence or absence of LPS stimulation. Bars represent the mean concentration of IL1β (a), TNF-α (b), IL6 (c) and IL10 (d) ± S.E.M (Two-way ANOVA test; *p < .05, **p < .01, ***p < .001). “-LPS” represents the concentrations of cytokines after 4 or 24 hours without LPS. Panels (e-h) show the concentrations of crucial cytokines (TNF-α and IL10) in the supernatants of immunomagnetically isolated monocytes from healthy donors (HD; n = 20; panels E, G) and MF patients (n = 20; panels F, H) in vitro cultured for 24 hours in the presence or absence of LPS stimulation and titrating doses of RUX (0.2–10 µM). Bars represent the mean concentration of TNF-α and IL10 ± S.E.M (one-way ANOVA test; *p < .05, **p < .01, ***p < .001).