Figure 1. Single-cell transcriptome of Tregs from fresh and cryopreserved/thawed PBMCs. (A) Assessment of the quality of scRNA-seq results. Captured UMI (left), number of gene species (middle), and proportion of mitochondrial transcript (right) in the fresh sample and cryopreserved/thawed sample. Data are given as the median value and 1st–3rd quartile range for each parameter. (B) UMAPs of 4,990 cells for the fresh sample and 4,495 cells for the cryopreserved/thawed sample, colored by cluster. (C) Normalized expression of cell cycle genes on UMAP plots, colored by stage of the cell cycle. (D-G) Normalized expression of representative marker genes from Tregs on UMAP plots. CD3E (CD3) (D), CD4 (E), IL2RA (CD25) (F), and FOXP3 (FoxP3) (G).
