Figure 2. Cluster-specific up-regulated genes in fresh and cryopreserved/thawed cells. (A) Heatmaps presenting up-regulated genes from each cluster for the fresh sample and (B) the cryopreserved/thawed sample. The arrows indicate the commonly up-regulated genes in the clusters from both fresh and cryopreserved/thawed samples. The pink box indicates cluster 4 in the cryopreserved/thawed sample, with multiple up-regulated heat shock protein genes. (C) Plots of the variable genes from the fresh or cryopreserved/thawed samples, or both. (D) UMAP plot of the representative expression pattern for HSPA1A.