Figure 6.

Effects of VC1 on plasma clotting induced by Ca²⁺. Plasma-citrate was preincubated with various amounts of VC1 (0.1-1.66 nmol in 100 µl of plasma) and the intrinsic pathway was activated by the addition of aPTT reagent (10 µl). After addition of 20 µl of 25 mm CaCl₂, plasma clotting was monitored by measuring the absorbance at 405 nm using a microtiter plate reader. Time course curves are representative of three independent experiments.