Table 2.

Identification of the 75-kDa polypeptide as prothrombin

MS fingerprinting of the ∼75 kDa band excised from the gel of the VC1-bound material fraction separated by SDS-PAGE as shown in Fig. 1A.

Peptide sequencea	PSMsb	XCorrc	Charged	MH+ [Da]e	ΔMf
					ppm
101AAcLEGNcAEGLGTNY116	2	3.64	2	1699.70293	−1.29
105EGNcAEGLGTNYR117	2	3.35	2	1440.61601	−0.88
178RQEcSIPVcGQDQVTVAmTPR198	2	6.49	3	2448.14023	−1.11
199SEGSSVNLSPPLEQcVPDR217	2	3.94	2	2070.97295	−1.55
207SPPLEQcVPDR217	2	3.00	2	1297.61797	−2.01
207SPPLEQcVPDRGQQY221	2	5.31	2	1773.82146	−0.60
248HQDFNSAVQLVENF261	2	3.75	2	1647.77519	−0.62
252NSAVQLVENF261	2	3.61	2	1120.56145	−1.71
276VAGKPGDFGY285	2	3.10	2	1010.49156	−2.63
289NYcEEAVEEETGDGLDEDSDR309	2	4.87	2	2431.93169	0.07
291cEEAVEEETGDGLDEDSDR309	2	5.11	2	2154.82280	−1.15
330GSGEADcGLRPL341	2	3.73	2	1231.57231	−1.07
364IVEGSDAEIGMSPW377	2	3.37	2	1490.67912	−2.77
391cGASLISDR399	2	2.95	2	978.46599	−1.40
418TENDLLVR425	2	3.17	2	959.51494	−0.77
435ERNIEKISML444	1	3.21	2	1232.66509	−1.39
475SDYIHPVcLPDR486	4	3.30	3	1471.69828	−1.09
478IHPVcLPDRETAASL492	2	3.25	2	1678.85649	−1.01
524QVVNLPIVER533	2	2.91	2	1166.68779	−1.24
524QVVNLPIVERPVcK537	4	3.87	2	1650.93450	−0.94
554KPDEGKR560	2	2.90	2	829.45293	0.29
560RGDAcEGDSGGPF572	2	4.19	2	1324.52056	−1.34
561GDAcEGDSGGPF572	2	3.24	2	1168.41997	−1.07
613IQKVIDQFGE622	2	3.69	2	1176.62395	−1.72

^aThe amino acid residues refer to the precursor sequence of prothrombin from Homo sapiens (UniProt accession number P00734, amino acids 1-622). Sequence coverage was 35.37%. Lowercase c indicates cysteine carbamidomethylation and lowercase m indicates methionine oxidation.

^bPSMs: peptide spectrum matches (total number of identified peptide sequences including those redundantly identified).

^cXCorr score values from Sequest algorithm.

^dCharge state of the precursor ion.

^eMH+ [Da] represents the singly charged mass of the precursor ions in Da.

^fDifference between the theoretical mass of the peptide and the experimental mass of the precursor ion.