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Disruption of Alcam gene exon 13 potentiates the shedding of endogenous ALCAM protein. RAW 264.7 cells expressing exon 13-sgRNA/Cas9 (exon 13 del) or Cas9 only (control) were treated with or without LPS and/or BB94 for 60 min, and cell extracts and culture supernatants were subjected to Western blotting (IB) using the indicated antibodies. β-Actin was used as a loading control. Black dots, nonspecific bands. Ext, extracellular domain. Cyto, cytoplasmic domain.
