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. 2020 Jul 1;295(35):12398–12407. doi: 10.1074/jbc.AC120.014189

Figure 2.

Figure 2.

AHR regulates the expression of enzymes in the de novo pyrimidine biosynthesis pathway in fibroblasts. A, schematic representation of the de novo and salvage pyrimidine biosynthesis pathways. B, heat map of the expression levels of CAD, DHODH, and UMPS in myc−/− and MYC cells 48 h after transfection with control or AHR siRNA. C, schematic representation of the rat CAD, DHODH, and UMPS gene promoter regions displaying the presence of XRE (AHR-binding sites) or E-box (MYC-binding sites). D, qPCR for AHR, CAD, DHODH, and UMPS in myc−/− cells expressing MYC or empty vector (E.V.) 72 h after transfection with control siRNA (siCtrl) or AHR siRNA (siAHR). E, Western blotting for the indicated proteins in myc−/− rat fibroblasts reconstituted with MYC 72 h after transfection with control or AHR siRNAs. F, Western blotting of the WT rat fibroblasts TGR1 72 h after transfection with control or AHR siRNAs. G, relative amounts of uridine levels found in the metabolomic screening, normalized to the myc−/− siCtrl conditions. H, schematic representation of the nucleoside transporters. I, relative mRNA levels of the nucleoside transporters found in our RNA-seq in myc−/− and MYC cells 48 h after transfection with control or AHR siRNA. Asterisks represent p values of <0.05.