Figure 1.
Differential regulation of gene expression by BA pool composition. A, simplified BA synthesis pathway. B, composition of BA pools used in this study. Glycine-conjugated BAs were used in human pools, whereas taurine-conjugated BAs were used in mouse pools, except LCA, which was unconjugated. C, expression of FXR target genes measured by qPCR. n = 3–4 wells of organoids/condition. D, genes preferentially induced by H10 and M10 compared with H90 and M90 identified by RNA-Seq (log2 -fold change > 1 and padj < 0.05). n = 3/condition. E, expression of VDR target genes measured by qPCR (n = 3–4 wells/condition). Full result tables for one-way ANOVA (C and E) and post hoc pairwise comparisons are shown in Table S4. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ns, not significant. Error bars, S.D.